- Title
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Epilepsy, Behavioral Abnormalities, and Physiological Comorbidities in Syntaxin-Binding Protein 1 (STXBP1) Mutant Zebrafish
- Authors
- Grone, B.P., Marchese, M., Hamling, K.R., Kumar, M.G., Krasniak, C.S., Sicca, F., Santorelli, F.M., Patel, M., Baraban, S.C.
- Source
- Full text @ PLoS One
Ontogeny of stxbp1a and stxbp1b expression in zebrafish larvae.Wild-type zebrafish were probed for expression of stxbp1a mRNA (A-E) or stxbp1b mRNA (F-J). Abbreviations: CeP: cerebellar plate, Ha: habenula, IR: inner retina, MO: medulla oblongata, OB: olfactory bulb, P: pallium, Ret: retina, SC: spinal cord, Tel: telencephalon, TeO: optic tectum, Scale bar = 100µm. EXPRESSION / LABELING:
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Morphology of stxbp1as3000 mutant zebrafish.(A) Heterozygous stxbp1as3000/+ mutant larvae (5 dpf) are morphologically indistinguishable from wild-type siblings. Homozygous stxbp1as3000/s3000 mutant larvae are immobile and fail to hatch out of the chorion. Scale bar = 500 µm. (B) Homozygous stxbp1as3000/s3000 mutant larvae (n = 10) removed from their chorions are not significantly different in length from their siblings (n = 30; p = 0.0592, two-tailed t-test). (C-D) At 5 dpf, the dorsal surface of homozygous stxbp1as3000/s3000 mutant larvae that were removed from their chorions at 2 dpf (D) show dispersed melanin and foreshortened craniofacial structure compared to siblings (C). Scale bar = 300 µm. PHENOTYPE:
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Morphology of stxbp1bs3001 mutant zebrafish.(A) Both heterozygous stxbp1bs3001/+ and homozygous stxbp1bs3001/s3001 mutant larvae (5 dpf) are morphologically similar to their wild-type siblings. Scale bar = 500 µm. (B) Homozygous stxbp1bs3001/s3001 mutant larvae (n = 8) are not significantly different in length from their siblings (n = 13) (p = 0.2297, two-tailed t-test). (C-D) The dorsal surface of homozygous stxbp1bs3001/s3001 mutant larvae (D) show dispersed melanin compared to siblings (C). Scale bar = 300 µm. PHENOTYPE:
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. |
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. |
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. PHENOTYPE:
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. PHENOTYPE:
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. PHENOTYPE:
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. PHENOTYPE:
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