|ZFIN ID: ZDB-PUB-970210-10|
Mutations affecting development of the notochord in zebrafish
Stemple, D.L., Solnica-Krezel, L., Zwartkruis, F., Neuhauss, S.C., Schier, A.F., Malicki, J., Stainier, D.Y., Abdelilah, S., Rangini, Z., Mountcastle-Shah, E., and Driever, W.
|Source:||Development (Cambridge, England) 123: 117-128 (Journal)|
|Registered Authors:||Abdelilah-Seyfried, Salim, Driever, Wolfgang, Malicki, Jarema, Mountcastle-Shah, Elizabeth, Neuhauss, Stephan, Schier, Alexander, Solnica-Krezel, Lilianna, Stainier, Didier, Stemple, Derek L.|
|Keywords:||zebrafish; notochord; floor plate; mesoderm; embryogenesis|
Stemple, D.L., Solnica-Krezel, L., Zwartkruis, F., Neuhauss, S.C., Schier, A.F., Malicki, J., Stainier, D.Y., Abdelilah, S., Rangini, Z., Mountcastle-Shah, E., and Driever, W. (1996) Mutations affecting development of the notochord in zebrafish. Development (Cambridge, England). 123:117-128.
ABSTRACTThe notochord is critical for the normal development of vertebrate embryos. It serves both as the major skeletal element of the embryo and as a signaling source for the establishment of pattern within the neurectoderm, the paraxial mesoderm and other tissues. In a large-scale systematic screen of mutations affecting embryogenesis in zebrafish we identified 65 mutations that fall into 29 complementation groups, each leading to a defect in the formation and/or maintenance of the notochord. These mutations produce phenotypic abnormalities at numerous stages of notochord development, thereby establishing a phenotypic pathway, which in turn suggests a genetic pathway for the development of the notochord. Perturbations within adjacent tissues in mutant embryos further indicate the importance of notochord-derived signals for patterning within the embryo and suggest that these mutations will yield additional insight into the cues that regulate these patterning processes.