PUBLICATION

Mutations affecting development of the notochord in zebrafish

Authors
Stemple, D.L., Solnica-Krezel, L., Zwartkruis, F., Neuhauss, S.C., Schier, A.F., Malicki, J., Stainier, D.Y., Abdelilah, S., Rangini, Z., Mountcastle-Shah, E., and Driever, W.
ID
ZDB-PUB-970210-10
Date
1996
Source
Development (Cambridge, England)   123: 117-128 (Journal)
Registered Authors
Abdelilah-Seyfried, Salim, Driever, Wolfgang, Malicki, Jarema, Mountcastle-Shah, Elizabeth, Neuhauss, Stephan, Schier, Alexander, Solnica-Krezel, Lilianna, Stainier, Didier, Stemple, Derek L.
Keywords
zebrafish; notochord; floor plate; mesoderm; embryogenesis
MeSH Terms
  • Animals
  • Brain/embryology
  • Cell Differentiation/genetics
  • Gene Expression Regulation, Developmental*
  • Mesoderm/physiology
  • Mutation*
  • Notochord/anatomy & histology
  • Notochord/cytology
  • Notochord/embryology*
  • Phenotype
  • Zebrafish/embryology*
  • Zebrafish/genetics*
PubMed
9007234 Full text @ Development
Abstract
The notochord is critical for the normal development of vertebrate embryos. It serves both as the major skeletal element of the embryo and as a signaling source for the establishment of pattern within the neurectoderm, the paraxial mesoderm and other tissues. In a large-scale systematic screen of mutations affecting embryogenesis in zebrafish we identified 65 mutations that fall into 29 complementation groups, each leading to a defect in the formation and/or maintenance of the notochord. These mutations produce phenotypic abnormalities at numerous stages of notochord development, thereby establishing a phenotypic pathway, which in turn suggests a genetic pathway for the development of the notochord. Perturbations within adjacent tissues in mutant embryos further indicate the importance of notochord-derived signals for patterning within the embryo and suggest that these mutations will yield additional insight into the cues that regulate these patterning processes.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping