PUBLICATION
Reversible Influence of Hemipiperazine Photochromism on the Early Development of Zebrafish Embryo
- Authors
- Seliwjorstow, A., Takamiya, M., Rastegar, S., Pianowski, Z.
- ID
- ZDB-PUB-240306-5
- Date
- 2024
- Source
- Chembiochem : a European journal of chemical biology 25(8): e202400143 (Journal)
- Registered Authors
- Rastegar, Sepand, Takamiya, Masanari
- Keywords
- Cytoskeleton dynamics, epiboly, hemipiperazine, photochromic antimitotics, zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian
- Gastrulation*/physiology
- Microtubules
- Tubulin/metabolism
- Zebrafish*/metabolism
- PubMed
- 38442077 Full text @ Chembiochem
Citation
Seliwjorstow, A., Takamiya, M., Rastegar, S., Pianowski, Z. (2024) Reversible Influence of Hemipiperazine Photochromism on the Early Development of Zebrafish Embryo. Chembiochem : a European journal of chemical biology. 25(8):e202400143.
Abstract
This study explores the potential of controlling organismal development with light by using reversible photomodulation of activity in bioactive compounds. Specifically, our research focuses on plinabulin 1, an inhibitor of tubulin dynamics that contains a photochromic motif called hemipiperazine. The two isomeric forms, Z-1 and E-1, can partially interconvert with light, yet show remarkable thermal stability in darkness. The Z-isomer exhibits higher cytotoxicity due to stronger binding to α-tubulin's colchicine site. The less toxic E-1 form, considered a "pro-drug", can be isolated in vitro and stored. Upon activation by blue or cyan light, it predominantly generates the more toxic Z-1 form. Here we demonstrate that 1 can effectively photomodulate epiboly, a critical microtubule-dependent cell movement during gastrulation in zebrafish embryos. This research highlights the potential of photomodulation for precise and reversible control of cellular activities and organismal development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping