PUBLICATION

Nano-bubble hydrogen water: An effective therapeutic agent against inflammation related disease caused by viral infection in zebrafish model

Authors
Li, C., Cao, Y., Kohei, F., Hao, H., Peng, G., Cheng, C., Ye, J.
ID
ZDB-PUB-220308-8
Date
2022
Source
Virologica Sinica   37(2): 277-283 (Journal)
Registered Authors
Keywords
Inflammation, Nano-bubble hydrogen water (nano-HW), Spring viraemia of carp virus (SVCV), Zebrafish
MeSH Terms
  • Inflammation/drug therapy
  • Rhabdoviridae Infections*/drug therapy
  • Water
  • Animals
  • Rhabdoviridae
  • Carps*
  • Zebrafish
  • Hydrogen/pharmacology
  • Hydrogen/therapeutic use
  • Fish Diseases*/drug therapy
(all 10)
PubMed
35249853 Full text @ Virol Sin
Abstract
Since the anti-inflammatory effect of hydrogen has been widely known, it was supposed that hydrogen could suppress tissue damage by inhibiting virus-related inflammatory reactions. However, hydrogen is slightly soluble in water, which leads to poor effect of oral hydrogen-rich water therapy. In this study, the nano-bubble hydrogen water (nano-HW) (about 0.7 ​ppm) was prepared and its therapeutic effect against viral infection was investigated by utilizing spring viraemia of carp virus (SVCV)-infected zebrafish as model. Three-month-old zebrafish were divided into nano-HW treatment-treated group and aquaculture water treated group (control group). The results revealed that the cumulative mortality rate of SVCV-infected zebrafish was reduced by 40% after treatment with nano-bubble hydrogen water, and qRT-PCR results showed that SVCV replication was significantly inhibited. Histopathological examination staining showed that SVCV infection caused tissue damage was greatly alleviated after treatment with nano-bubble hydrogen water. Futhermore, SVCV infection caused reactive oxygen species (ROS) accumulation was significantly reduced upon nano-HW treatment. The level of proinflammatory cytokines IL-1β, IL-8, and TNF-α was remarkably reduced in the nano-HW-treated group in vivo and in vitro. Taken together, our data demonstrated for the first time that nano-HW could inhibit the inflammatory response caused by viral infection in zebrafish, which suggests that nano-HW can be applied to antiviral research，and provides a novel therapeutic strategy for virus-caused inflammation related disease.
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Citation
Li, C., Cao, Y., Kohei, F., Hao, H., Peng, G., Cheng, C., Ye, J. (2022) Nano-bubble hydrogen water: An effective therapeutic agent against inflammation related disease caused by viral infection in zebrafish model. Virologica Sinica. 37(2):277-283.