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Fig. 4 Fig. 4. Effects of nano-HW on SVCV-Induced ROS production in zebrafish. ATotal reactive oxygen species were measured in mixed tissues (kidney, intestine, brain, liver and spleen) at 1, 3, 5, and 7 dpi with 2, 7-dichlorofuorescin diacetate (DCFH-DA) probes. B Superoxide anion was monitored in mixed tissues (kidney, intestine, brain, liver and spleen) using dihydroethidium (DHE) at 1, 3, 5, and 7 dpi. Each value was represented as mean ?± ?SEM of three fish. ?P ?< ?0.05, ??P ?< ?0.01, ???P ?< ?0.001, ????P ?< ?0.0001. ns means no significant difference.
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