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Fig. 6 Fig. 6. Effect of nano-HW on the inflammatory response in SVCV- infected ZF4 cells. ZF4 cells were cultured in DMEM/F-12 medium or nano-HW-prepared DMEM-F12 medium to reach to a logarithmic growth. ZF4 cells were infected with SVCV (1 MOI), and the mRNA expression levels of IL-1? (A), IL-8 (B), and TNF-? (C) were quantified by qRT-PCR at 12 hpi. The SVCV titer (D) of infected ZF4 cells was determined at 6, 12, 24, and 36 hpi. All data are representatives of three independent experiments. ?P ?< ?0.05, ??P ?< ?0.01, ???P ?< ?0.001, ????P ?< ?0.0001. ns means no significant difference.
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