Fig. 2 Fig. 2. Antiviral activity of nano-HW against SVCV in zebrafish. A Survival of zebrafish in the presence and absence of HW treatment. Size- and age-matched zebrafish (0.6 ?± ?0.1 ?g) were injected with 10 ??L of SVCV suspension (?2 ?× ?108 ?PFU/mL) per fish, and then treated with nano-HW. B Viral gene expression in nano-HW-treated and untreated zebrafish. The relative mRNA expression of SVCV G protein was examined by qRT-PCR in mixed tissues from 3 fish on 1, 3, 5 and 7 dpi. C The virus titer in nano-HW-treated and untreated zebrafish. The viral titer was measured by standard plaque assay with EPC cells. Viral particles in mixed tissues were titered on days 1, 3, 5, and 7 post-infection. Each value was represented as mean ?± ?SEM of three fishes. ?P ?< ?0.05, ??P ?< ?0.01, ???P ?< ?0.001, ????P ?< ?0.0001. ns means no significant difference.
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