ZFIN ID: ZDB-PUB-200620-15
Pax6 organizes the anterior eye segment by guiding two distinct neural crest waves
Takamiya, M., Stegmaier, J., Kobitski, A.Y., Schott, B., Weger, B.D., Margariti, D., Cereceda Delgado, A.R., Gourain, V., Scherr, T., Yang, L., Sorge, S., Otte, J.C., Hartmann, V., van Wezel, J., Stotzka, R., Reinhard, T., Schlunck, G., Dickmeis, T., Rastegar, S., Mikut, R., Nienhaus, G.U., Strähle, U.
Date: 2020
Source: PLoS Genetics   16: e1008774 (Journal)
Registered Authors: Dickmeis, Thomas, Gourain, Victor, Mikut, Ralf, Otte, Jens, Rastegar, Sepand, Strähle, Uwe, Takamiya, Masanari, Weger, Benjamin
Keywords: none
MeSH Terms:
  • Animals
  • Anterior Eye Segment/cytology
  • Anterior Eye Segment/embryology
  • Anterior Eye Segment/metabolism*
  • Cell Movement
  • Mutation
  • Neural Crest/cytology
  • Neural Crest/embryology
  • Neural Crest/metabolism*
  • Neurogenesis*
  • Neurons/cytology
  • Neurons/metabolism
  • PAX6 Transcription Factor/genetics
  • PAX6 Transcription Factor/metabolism*
  • Signal Transduction
  • Transforming Growth Factor beta/metabolism
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 32555736 Full text @ PLoS Genet.
Cranial neural crest (NC) contributes to the developing vertebrate eye. By multidimensional, quantitative imaging, we traced the origin of the ocular NC cells to two distinct NC populations that differ in the maintenance of sox10 expression, Wnt signalling, origin, route, mode and destination of migration. The first NC population migrates to the proximal and the second NC cell group populates the distal (anterior) part of the eye. By analysing zebrafish pax6a/b compound mutants presenting anterior segment dysgenesis, we demonstrate that Pax6a/b guide the two NC populations to distinct proximodistal locations. We further provide evidence that the lens whose formation is pax6a/b-dependent and lens-derived TGFβ signals contribute to the building of the anterior segment. Taken together, our results reveal multiple roles of Pax6a/b in the control of NC cells during development of the anterior segment.