PUBLICATION
Intravital correlated microscopy reveals differential macrophage and microglial dynamics during resolution of neuroinflammation
- Authors
- van Ham, T.J., Brady, C.A., Kalicharan, R.D., Oosterhof, N., Kuipers, J., Veenstra-Algra, A., Sjollema, K.A., Peterson, R.T., Kampinga, H.H., Giepmans, B.N.
- ID
- ZDB-PUB-140629-3
- Date
- 2014
- Source
- Disease models & mechanisms 7: 857-869 (Journal)
- Registered Authors
- Peterson, Randall, van Ham, Tjakko
- Keywords
- Brain, Intravital microscopy, Leukocytes, Microglia, Neurodegeneration, Zebrafish
- MeSH Terms
-
- Microscopy/methods*
- Inflammation/pathology*
- Astrocytes/pathology
- Microglia/pathology*
- Microglia/ultrastructure
- Time Factors
- Animals
- Cell Count
- Membrane Glycoproteins/metabolism
- Green Fluorescent Proteins/metabolism
- Phagocytes/pathology
- Phagocytes/ultrastructure
- Macrophages/pathology*
- Neurons/pathology
- Phagocytosis
- Brain/pathology*
- Brain/ultrastructure
- Microfilament Proteins/metabolism
- Larva
- Zebrafish
- Apolipoproteins E/metabolism
- Neutrophils/pathology
- Cell Death
- PubMed
- 24973753 Full text @ Dis. Model. Mech.
Citation
van Ham, T.J., Brady, C.A., Kalicharan, R.D., Oosterhof, N., Kuipers, J., Veenstra-Algra, A., Sjollema, K.A., Peterson, R.T., Kampinga, H.H., Giepmans, B.N. (2014) Intravital correlated microscopy reveals differential macrophage and microglial dynamics during resolution of neuroinflammation. Disease models & mechanisms. 7:857-869.
Abstract
Many brain diseases involve activation of resident and peripheral immune cells to clear damaged and dying neurons. Which immune cells respond in what way to cues related to brain disease, however, remains poorly understood. To elucidate these in vivo immunological events in response to brain cell death we used genetically targeted cell ablation in zebrafish. Using intravital microscopy and large-scale electron microscopy, we defined the kinetics and nature of immune responses immediately following injury. Initially, clearance of dead cells occurs by mononuclear phagocytes, including resident microglia and macrophages of peripheral origin, whereas amoeboid microglia are exclusively involved at a later stage. Granulocytes, on the other hand, do not migrate towards the injury. Remarkably, following clearance, phagocyte numbers decrease, partly by phagocyte cell death and subsequent engulfment of phagocyte corpses by microglia. Here, we identify differential temporal involvement of microglia and peripheral macrophages in clearance of dead cells in the brain, revealing the chronological sequence of events in neuroinflammatory resolution. Remarkably, recruited phagocytes undergo cell death and are engulfed by microglia. Because adult zebrafish treated at the larval stage lack signs of pathology, it is likely that this mode of resolving immune responses in brain contributes to full tissue recovery. Therefore, these findings suggest that control of such immune cell behavior could benefit recovery from neuronal damage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping