PUBLICATION
Bone Regenerates via Dedifferentiation of Osteoblasts in the Zebrafish Fin
- Authors
- Knopf, F., Hammond, C., Chekuru, A., Kurth, T., Hans, S., Weber, C.W., Mahatma, G., Fisher, S., Brand, M., Schulte-Merker, S., and Weidinger, G.
- ID
- ZDB-PUB-110524-34
- Date
- 2011
- Source
- Developmental Cell 20(5): 713-724 (Journal)
- Registered Authors
- Brand, Michael, Chekuru, Avinash, Fisher, Shannon, Hammond, Chrissy, Hans, Stefan, Knopf, Franziska, Schulte-Merker, Stefan, Weidinger, Gilbert
- Keywords
- none
- MeSH Terms
-
- Animal Fins/cytology
- Animal Fins/metabolism*
- Animals
- Bone and Bones/cytology
- Bone and Bones/metabolism*
- Cell Dedifferentiation
- Down-Regulation
- Osteoblasts/cytology
- Osteoblasts/metabolism*
- Zebrafish
- PubMed
- 21571227 Full text @ Dev. Cell
Citation
Knopf, F., Hammond, C., Chekuru, A., Kurth, T., Hans, S., Weber, C.W., Mahatma, G., Fisher, S., Brand, M., Schulte-Merker, S., and Weidinger, G. (2011) Bone Regenerates via Dedifferentiation of Osteoblasts in the Zebrafish Fin. Developmental Cell. 20(5):713-724.
Abstract
While mammals have a limited capacity to repair bone defects, zebrafish can completely regenerate amputated bony structures of their fins. Fin regeneration is dependent on formation of a blastema, a progenitor cell pool accumulating at the amputation plane. It is unclear which cells the blastema is derived from, whether it forms by dedifferentiation of mature cells, and whether blastema cells are multipotent. We show that mature osteoblasts dedifferentiate and form part of the blastema. Osteoblasts downregulate expression of intermediate and late bone differentiation markers and induce genes expressed by bone progenitors. Dedifferentiated osteoblasts proliferate in a FGF-dependent manner and migrate to form part of the blastema. Genetic fate mapping shows that osteoblasts only give rise to osteoblasts in the regenerate, indicating that dedifferentiation is not associated with the attainment of multipotency. Thus, bone can regenerate from mature osteoblasts via dedifferentiation, a finding with potential implications for human bone repair.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping