PUBLICATION

Bone Regenerates via Dedifferentiation of Osteoblasts in the Zebrafish Fin

Authors
Knopf, F., Hammond, C., Chekuru, A., Kurth, T., Hans, S., Weber, C.W., Mahatma, G., Fisher, S., Brand, M., Schulte-Merker, S., and Weidinger, G.
ID
ZDB-PUB-110524-34
Date
2011
Source
Developmental Cell   20(5): 713-724 (Journal)
Registered Authors
Brand, Michael, Chekuru, Avinash, Fisher, Shannon, Hammond, Chrissy, Hans, Stefan, Knopf, Franziska, Schulte-Merker, Stefan, Weidinger, Gilbert
Keywords
none
MeSH Terms
  • Cell Dedifferentiation
  • Osteoblasts/cytology
  • Osteoblasts/metabolism*
  • Animals
  • Zebrafish
  • Down-Regulation
  • Animal Fins/cytology
  • Animal Fins/metabolism*
  • Bone and Bones/cytology
  • Bone and Bones/metabolism*
(all 10)
PubMed
21571227 Full text @ Dev. Cell
Abstract
While mammals have a limited capacity to repair bone defects, zebrafish can completely regenerate amputated bony structures of their fins. Fin regeneration is dependent on formation of a blastema, a progenitor cell pool accumulating at the amputation plane. It is unclear which cells the blastema is derived from, whether it forms by dedifferentiation of mature cells, and whether blastema cells are multipotent. We show that mature osteoblasts dedifferentiate and form part of the blastema. Osteoblasts downregulate expression of intermediate and late bone differentiation markers and induce genes expressed by bone progenitors. Dedifferentiated osteoblasts proliferate in a FGF-dependent manner and migrate to form part of the blastema. Genetic fate mapping shows that osteoblasts only give rise to osteoblasts in the regenerate, indicating that dedifferentiation is not associated with the attainment of multipotency. Thus, bone can regenerate from mature osteoblasts via dedifferentiation, a finding with potential implications for human bone repair.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hu4008TgTransgenic Insertion
    kca66TgTransgenic Insertion
      tud7TgTransgenic Insertion
        tud8TgTransgenic Insertion
          tud9TgTransgenic Insertion
            zf131TgTransgenic Insertion
              zf132TgTransgenic Insertion
                zf259TgTransgenic Insertion
                  1 - 8 of 8
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  No data available
                  Fish
                  Fish
                  hu4008Tg
                  hu4008Tg; tud7Tg
                  hu4008Tg; zf131Tg
                  kca66Tg
                  tud7Tg
                  tud8Tg
                  tud9Tg
                  WT
                  zf131Tg
                  zf132Tg
                  1 - 10 of 11
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                  Antibodies
                  Name Type Antigen Genes Isotypes Host Organism
                  zns-5monoclonal
                    		IgG1Mouse
                    1 - 1 of 1
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                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    EGFPEFGEGFP
                    GFPEFGGFP
                    mCherryEFGmCherry
                    1 - 3 of 3
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                    Mapping
                    No data available
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                    Citation
                    Knopf, F., Hammond, C., Chekuru, A., Kurth, T., Hans, S., Weber, C.W., Mahatma, G., Fisher, S., Brand, M., Schulte-Merker, S., and Weidinger, G. (2011) Bone Regenerates via Dedifferentiation of Osteoblasts in the Zebrafish Fin. Developmental Cell. 20(5):713-724.