PUBLICATION

Graded levels of Ptf1a differentially regulate endocrine and exocrine fates in the developing pancreas

Authors
Dong, P.D., Provost, E., Leach, S.D., and Stainier, D.Y.
ID
ZDB-PUB-081007-1
Date
2008
Source
Genes & Development   22(11): 1445-1450 (Journal)
Registered Authors
Dong, P. Duc, Leach, Steven D., Stainier, Didier
Keywords
Ptf1a, diabetes, endocrine, exocrine, pancreas
MeSH Terms
  • Animals
  • Gene Expression Regulation, Developmental*
  • Islets of Langerhans/embryology*
  • Pancreas/embryology*
  • Pancreas/metabolism
  • Pancreas, Exocrine/embryology*
  • Transcription Factors/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed
18519637 Full text @ Genes & Dev.
Abstract
The mechanisms regulating pancreatic endocrine versus exocrine fate are not well defined. By analyzing the effects of Ptf1a partial loss of function, we uncovered novel roles for this transcription factor in determining pancreatic fates. In a newly identified hypomorphic ptf1a mutant, pancreatic cells that would normally express ptf1a and become exocrine cells, express the endocrine marker Isl1, indicating a cell fate switch. Surprisingly, a milder reduction of Ptf1a leads to an even greater increase of ectopic endocrine cells, suggesting that Ptf1a also plays a role in promoting endocrine development. We propose that low levels of Ptf1a promote endocrine fate, whereas high levels repress endocrine fate and promote exocrine fate.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes