ZFIN ID: ZDB-PUB-050623-1
nanor, a novel zygotic gene, is expressed initially at the midblastula transition in zebrafish
Bree, R.T., McLoughlin, S., Jin, S.W., McMeel, O.M., Stainier, D.Y., Grealy, M., and Byrnes, L.
Date: 2005
Source: Biochemical and Biophysical Research Communications 333(3): 722-728 (Journal)
Registered Authors: Bree, Ronan, Byrnes, Lucy, Jin, Suk-Won, McLoughlin, Sarah, Stainier, Didier
Keywords: Gene expression; Midblastula transition; Nanor; SSH; Zebrafish
MeSH Terms: Animals; Base Sequence; Blastula/metabolism*; Carrier Proteins/genetics*; Casein Kinase II/metabolism (all 15) expand
PubMed: 15961062 Full text @ Biochem. Biophys. Res. Commun.
FIGURES   (current status)
ABSTRACT
A novel, developmentally regulated gene, nanor, was identified by suppression subtractive hybridization. It is first expressed following the midblastula transition (MBT), a critical developmental stage in the early vertebrate embryo when the zygotic genome is activated. The nanor cDNA (626bp) includes a complete open reading frame but neither the gene nor the deduced amino acid sequence shows significant similarity to any known gene or protein. Nanor encodes a 175 amino acid putative protein with a protein kinase C and three casein kinase II phosphorylation sites, an N-myristoylation site and an NFX-type zinc-finger domain, indicating a potential role in transcriptional regulation. Semi-quantitative RT-PCR, Northern blot, and in situ hybridization analysis revealed that nanor expression is developmentally regulated. It is initially expressed after the MBT at the sphere stage and during epiboly it is expressed in the forerunner cells. At 24h post-fertilization, expression is solely anterior.
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