PUBLICATION

nanor, a novel zygotic gene, is expressed initially at the midblastula transition in zebrafish

Authors
Bree, R.T., McLoughlin, S., Jin, S.W., McMeel, O.M., Stainier, D.Y., Grealy, M., and Byrnes, L.
ID
ZDB-PUB-050623-1
Date
2005
Source
Biochemical and Biophysical Research Communications   333(3): 722-728 (Journal)
Registered Authors
Bree, Ronan, Byrnes, Lucy, Jin, Suk-Won, McLoughlin, Sarah, Stainier, Didier
Keywords
Gene expression; Midblastula transition; Nanor; SSH; Zebrafish
MeSH Terms
  • Animals
  • Base Sequence
  • Blastula/metabolism*
  • Carrier Proteins/genetics*
  • Casein Kinase II/metabolism
  • DNA Primers
  • DNA, Complementary
  • Gene Expression Regulation, Developmental*
  • Molecular Sequence Data
  • Phosphorylation
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics*
  • Zygote/metabolism*
PubMed
15961062 Full text @ Biochem. Biophys. Res. Commun.
Abstract
A novel, developmentally regulated gene, nanor, was identified by suppression subtractive hybridization. It is first expressed following the midblastula transition (MBT), a critical developmental stage in the early vertebrate embryo when the zygotic genome is activated. The nanor cDNA (626bp) includes a complete open reading frame but neither the gene nor the deduced amino acid sequence shows significant similarity to any known gene or protein. Nanor encodes a 175 amino acid putative protein with a protein kinase C and three casein kinase II phosphorylation sites, an N-myristoylation site and an NFX-type zinc-finger domain, indicating a potential role in transcriptional regulation. Semi-quantitative RT-PCR, Northern blot, and in situ hybridization analysis revealed that nanor expression is developmentally regulated. It is initially expressed after the MBT at the sphere stage and during epiboly it is expressed in the forerunner cells. At 24h post-fertilization, expression is solely anterior.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes