PUBLICATION
A single-cell-resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle
- Authors
- Yang, Y., Wang, H., He, J., Shi, W., Jiang, Z., Gao, L., Jiang, Y., Ni, R., Yang, Q., Luo, L.
- ID
- ZDB-PUB-210624-7
- Date
- 2021
- Source
- Proceedings of the National Academy of Sciences of the United States of America 118(25): (Journal)
- Registered Authors
- Luo, Lingfei
- Keywords
- cell cycle, endoderm, fate map, pancreatic fate, single-cell labeling and tracing
- MeSH Terms
-
- Animals
- Cell Cycle*
- Cell Lineage
- Cell Proliferation
- Endoderm/cytology*
- G1 Phase
- Hedgehog Proteins/metabolism
- Pancreas/cytology*
- Signal Transduction
- Single-Cell Analysis*
- Stem Cells/cytology*
- Zebrafish/embryology*
- Zebrafish Proteins/metabolism
- PubMed
- 34161274 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Yang, Y., Wang, H., He, J., Shi, W., Jiang, Z., Gao, L., Jiang, Y., Ni, R., Yang, Q., Luo, L. (2021) A single-cell-resolution fate map of endoderm reveals demarcation of pancreatic progenitors by cell cycle. Proceedings of the National Academy of Sciences of the United States of America. 118(25):.
Abstract
A progenitor cell could generate a certain type or multiple types of descendant cells during embryonic development. To make all the descendant cell types and developmental trajectories of every single progenitor cell clear remains an ultimate goal in developmental biology. Characterizations of descendant cells produced by each uncommitted progenitor for a full germ layer represent a big step toward the goal. Here, we focus on early foregut endoderm, which generates foregut digestive organs, including the pancreas, liver, foregut, and ductal system, through distinct lineages. Using unbiased single-cell labeling techniques, we label every individual zebrafish foregut endodermal progenitor cell out of 216 cells to visibly trace the distribution and number of their descendant cells. Hence, single-cell-resolution fate and proliferation maps of early foregut endoderm are established, in which progenitor regions of each foregut digestive organ are precisely demarcated. The maps indicate that the pancreatic endocrine progenitors are featured by a cell cycle state with a long G1 phase. Manipulating durations of the G1 phase modulates pancreatic progenitor populations. This study illustrates foregut endodermal progenitor cell fate at single-cell resolution, precisely demarcates different progenitor populations, and sheds light on mechanistic insights into pancreatic fate determination.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping