IMAGE

Fig. 1

ID

ZDB-IMAGE-260710-1

Genes

Antibodies

Ab1-psa-ncam

Publication

Tsuji et al., 2026 - Neu1 deficiency is associated with reduced nicotine responsiveness and dopamine D3 receptor downregulation in zebrafish

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Figures for Tsuji et al., 2026

Image

Figure Caption

Fig. 1

Alterations in the levels of polysialic acid following nicotine exposure in zebrafish brain. A–C Representative lectin blot and immunoblot analyses of zebrafish brain extracts following Nco exposure. A α2,3-linked sialylated glycoproteins detected using lectin from Maackia amurensis (MAM), B α2,6-linked sialylated glycoproteins detected using lectin from Sambucus sieboldiana (SSA), and (C) PSA levels detected by anti-PSA antibody. β-Actin was used as a loading control. The intensities of PSA and β-Actin bands in (C) were quantified, and the results are presented as PSA/β-Actin levels relative to the values in the non-treated zebrafish. n = 5 for each group. D, E Relative mRNA expression levels of (D) neu1 and (E) PSA biosynthesis-related genes (st8sia2, st8sia4, ncam1a, and ncam1b) in zebrafish brains following Nco exposure. Expression levels are normalized to those of ef1α and expressed relative to the vehicle-treated controls. n = 6. n.s., not significant

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Fish Physiol. Biochem.