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Fig. 4 Ectopic expression of proneural cluster-related genes in her6 mutants. Offspring of heterozygotic crosses were stained by WISH at the bud stage (neurog1, dla, her4.1), photographed, and then genotypes. Dorsal views are shown with anterior to the top. The expression patterns in whole embryos (top) and hindbrain regions boxed above (bottom) are shown for respective genes and genotypes. Arrowheads indicate enhanced expression in the anterior-most portion of the neural plate (anb). Arrows (black and white) mark ectopic expression in r3 (m-IPCD-r3, l-IPCD-r3) and midline (md-IPCD-r3/5), respectively. Asterisks (*) indicate ectopic expression around drc and vrc and enhanced expression in vcc. Numbers of the embryos with the indicated expression patterns and total scored embryos are shown in the bottom-right. See the legend to Fig. 1 for abbreviations. Scale bars, 100 μm.