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Fig. 3 Splice morpholino of PEX1 increases the number of GABAergic KA” neurons. Expression of GAD1 and islet1 by whole-mount in situ hybridization staining in the embryos injected with Con-MO (A, C) and PEX1-MO (B, D) at 25 hpf. Dorsal to the top and anterior to the left. Arrowheads indicate KA” neurons between the 8th and the 10th somite (A, B). Arrows indicate motor neurons in the p3 domain (D). Expression of zn5/DM-GRASP was detected in the red channel of confocal images in Tg[olig2:egfp] embryos injected with Con-MO (E) and PEX1-MO (F) at 27 hpf. Filled arrowheads identify secondary motor neuron (sMN) in the pMN domain of Con-MO-injected Tg[olig2:egfp] embryos (E), and open arrows identify ectopic sMNs in the P3 domain of PEX1-MO-injected embryos (F). Cross-sectional views of a’ and b’, at the position of the dashed lines, are displayed in a and b, respectively. Scale bars: 25 µm.