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Chen et al., 2024 - Deletion of ddx4 Ovary-Specific Transcript Causes Dysfunction of Meiosis and Derepress of DNA Transposons in Zebrafish Ovaries

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Figure 4

ddx4-L knockout leads to significant downregulation of sycp1 at mRNA and protein levels. (A) Expression levels of several meiosis-related genes in WT and ddx4-E4Δ ovaries based on the RNA-seq data. The FPKM values are shown. n = 3. (B) qPCR validation of the mRNA levels of meiosis-related genes in WT and ddx4-E4Δ ovaries at 6 mpf. n = 3. (C) Detection of Sycp1 protein levels in WT and ddx4-E4Δ ovaries at 6 mpf by Western blot assay. RNA-seq tracks of sycp1 gene in WT and ddx4-E4Δ ovaries are shown in the top panel. Original western blot images are shown in Figure S1A. (D) Quantitative analysis of the bands in (C) shows the changes in Sycp1 protein levels in ddx4-E4Δ ovaries. n = 3.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biology (Basel)