ZFIN Image: Chen et al., 2024, Figure 4

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Figure Caption

Figure 4

ddx4-L knockout leads to significant downregulation of sycp1 at mRNA and protein levels. (A) Expression levels of several meiosis-related genes in WT and ddx4-E4Δ ovaries based on the RNA-seq data. The FPKM values are shown. n = 3. (B) qPCR validation of the mRNA levels of meiosis-related genes in WT and ddx4-E4Δ ovaries at 6 mpf. n = 3. (C) Detection of Sycp1 protein levels in WT and ddx4-E4Δ ovaries at 6 mpf by Western blot assay. RNA-seq tracks of sycp1 gene in WT and ddx4-E4Δ ovaries are shown in the top panel. Original western blot images are shown in Figure S1A. (D) Quantitative analysis of the bands in (C) shows the changes in Sycp1 protein levels in ddx4-E4Δ ovaries. n = 3.

Acknowledgments

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