|
Fig. 6 Ombuoside protects zebrafish hair cells from cisplatin-induced damage. A?D. Tg(brn3c:GFP) Zebrafish larvae at 5 dpf were pre-exposed in ombuoside for 2 h, and then co-treated with 800 ?mol/L cisplatin for 8 h. Confocal microscopy was used to detect the green fluorescent protein (GFP) expression of hair cells, N ? 8. E. Assessment of the number of hair cells per neuromast in Fig. .6A?D, N ? 8.F. Relative expression of apoptosis-associated mRNA in zebrafish treated with cisplatin alone or co-treated with ombuoside, N = 3. Data are presented as mean ± SEM. ?P ? 0.05, ??P ? 0.01, ???P ? 0.001, and ????P ? 0.0001. Scale bar: 10 ?m.