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Figure 7 Pseudobulk differential expression analysis of single-cell RNAseq data. (A) Heatmaps showing the 50 most differentially expressed genes (DEGs) in 3 major cell types; ectoderm (clusters 4, 5, 6, 7), paraxial mesoderm (clusters 10, 11, 12), and lateral plate mesoderm (clusters 15, 16, 17,18) between dact1/2?/? mutants and wildtype and gpc4?/? mutants and wildtype. (B) Venn diagrams showing unique and overlapping DEGs in dact1/2?/? and gpc4?/? mutants. (C) Gene Ontology (GO) analysis of dact1/2?/? mutant-specific DEGs in ectoderm showing enrichment for proteolytic processes.
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