Figure 1.

Figure 1.

Localization of mglur6-pathway genes labeled by fluorescence in situ hybridization. (A–F) Z-stacks of two to three images of adult zebrafish retinal slices taken with a confocal microscope. (A′–F′) Genes in A–F are coexpressed in the same cell body as shown by differential interference contrast (DIC) images as background. (A, A′) The arrowheads point to bipolar cell soma where mglur6b and mglur6a colocalize. While mglur6a labeling is restricted to the medial inner nuclear layer (INL), the mglur6b riboprobe additionally labels cell bodies in the proximal INL (arrows). (B, B′) The α-subunit of the G-protein (gnaob) is located in the same ON-bipolar cells as mglur6a (arrowheads). (C, C′) mglur6a colocalizes with trpm1a in ON-bipolar cells (arrowheads), but for some cell bodies, it is unclear whether exactly the same soma or two different cell bodies next to each other are labeled (arrow). (D, D′) The mglur6b riboprobe is expressed in the same cells where trpm1a is located (arrowheads). (E, E′) The cation channel trpm1a colocalizes with nyx in green in the medial INL (arrowheads). (F, F′) Both trpm1 channel genes are expressed in the INL. While trpm1a clearly labels ON-bipolar cell soma (arrowheads), trpm1b is only marginally expressed in the medial INL but rather labels structures in the proximal INL. All images reveal the whole INL, as depicted in D. Scale bars: 10 µm. Scale bars differ in each figure, so they are individually labeled.