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Fig. 4 No alterations in other definitive hematopoietic lineages in epoaszy8. (A and B) cmyb WISH (A, indicated by red triangular arrowheads) identified HSPCs at 36-hpf in epoaszy8 compared with WT (Fisher's exact test, n ? 8, ns: no significance). (C and D) lyz WISH (C, indicated by red triangular arrowheads) identified granulocytes at 3-dpf in epoaszy8 compared with WT (Student's t-test, n > 8, mean ± SD, ns: no significance). (E and F) mfap4 WISH (E, indicated by red triangular arrowheads) identified macrophages at 4-dpf in epoaszy8 compared with WT (Student's t-test, n > 6, mean ± SD, ns: no significance). (G and H) mpl WISH (G, indicated by red triangular arrowheads) identified thrombocytes at 4-dpf in epoaszy8 compared with WT (Student's t-test, n > 15, mean ± SD, ns: no significance). (I and J) rag1 WISH (I, indicated by red triangular arrowheads) identified lymphocytes at 5-dpf in epoaszy8 compared with WT (Student's t-test, n > 6, replicates = 3, mean ± SEM, ns: no significance).