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Li et al., 2024 - Defining morphologically and genetically distinct GAGBergic/cholinergic amacrine cell subtypes in the vertebrate retina

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Fig 1 Single-cell RNA-seq identifies 2 GABAergic/cholinergic AC types.

(A) t-SNE plot showing 5 clusters of ACs (in colors) in single-cell RNA-seq data. (B) Expression pattern of marker genes for each cluster in (A). Marker genes gad1b (GABAergic), vachta (cholinergic), sox2 (AC5), bhlhe22 (AC4), glyt1 (AC3), gad2 (AC2), and sox4a (AC1) are highlighted in red. (C-F) Validation of marker TFs for cluster AC4 (C and D) and AC5 (E and F) using in situ hybridization (magenta) combined with ChAT immunostaining (green) of the 5-dpf zebrafish. Dashed circles, ACs with positive signals. Scale bars, 10 μm. AC, amacrine cell; ChAT, choline acetyltransferase; dpf, days post-fertilization; GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; TF, transcription factor.

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