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Fig. 5 Posterior arch skeletogenic CNCCs do not differentiate in nkx2.3 morphants. (A) Detection of Col2a proteins in the pharyngeal arches at indicated stages. Embryos of Tg(fli1:EGFP) injected with nkx2.3 MOs were co-immunostained with anti-GFP (green) and anti-Col2a (red) antibodies. The palatoquadrate (pq), ethmoid plate (ep) and ceratohyal (ch) cartilages were shown with anterior to the left. Scale bars, 20 μm. (B) The expression level of marker genes of CNCCs’ differentiation was detected by WISH at 72 hpf.
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