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Shanaka et al., 2023 - Viperin mutation is linked to immunity, immune cell dynamics, and metabolic alteration during VHSV infection in zebrafish

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Figure 3

Neutrophil and macrophage recruitment in viperin^-/- fish. (A) Zebrafish lines viperin^-/-Tg(mpx:mcherry) and viperin^-/-Tg(mpeg:egfp) were generated to track neutrophils and macrophages, respectively. The caudal fin was amputated of these lines and immersed in poly I:C. (B) Analysis of neutrophil recruitment, (C) the recruitment of the macrophages to the site of infection was observed in a time-dependent manner (I-Wt PBS, II-Wt+Poly I:C, III-viperin^-/- + PBS, and IV-viperin^-/- + Poly I:C). Dynamics of the neutrophils (D), macrophages (E) at the wound site showed time-dependent differences between the WT and viperin^-/- fish. Expression analysis of neutrophil marker genes mpx(F) and nox(G) in the adult zebrafish with VHSV infection showed significant upregulation of the marker genes in viperin^-/- fish compared to those in WT fish. Gene expression analysis for the marker gene csf1a(H) for macrophages, indicating significant downregulation in viperin^-/- fish. Poly I:C, Polyinosinic:polycytidylic acid; hpi, Hours post infection; mpx, myeloid-specific peroxidase; nox, NADPH oxidase; csf1a, colony stimulating factor 1.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol