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Fig. 3
Upregulation of Nrf2-target genes in keap1a;keap1b-DKO larvae. (A) WISH analysis of gstp1.2 expression in 5-dpf offspring from Ka0Kb1N1 parents. The arrowhead indicates the gill. The numbers in each picture indicate the numbers of larvae with similar staining profiles and tested larvae. (B) qRT-PCR analysis of the relative expressions of gstp1.2, prdx1, txn, and gss in wild-type, Ka0Kb0N2, and Ka0Kb0N0 larvae at 5 dpf. The expression of the wild-type specimens was normalized to 1. Asterisks indicate significant differences (*p < 0.05, **p < 0.01).
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