IMAGE

Fig. 10

ID

ZDB-IMAGE-230807-10

Genes

gsx1
gsx2

Publication

Coltogirone et al., 2022 - Gsx2, but not Gsx1, is necessary for early forebrain patterning and long-term survival in zebrafish

All Figures

Figures for Coltogirone et al., 2022

Image

Figure Caption

Fig. 10 gsx1 and gsx2 expression in gsx1 and gsx2 mutant zebrafish. (Aⁱ-A^iv) whole-mount in situ hybridization (WISH) images at 30 hpf showing gsx1 expression in wild-type and gsx2^y691 embryos (top) and gsx2 expression in wild-type and gsx1^y689 embryos. Images are ×20 compound scope images with embryos mounted under cover glass, eyes dissected, and anterior facing left. (Bⁱ-B^iv) FISH images at 30 hpf. Images are ×20 confocal images with embryos mounted under cover glass, eyes dissected, and anterior facing left. (C) FIJI-ImageJ quantification of images in (Aⁱ-A^iv). (D) FIJI-ImageJ quantification of images in (Bⁱ-B^iv). Sample sizes for all FIJI-ImageJ analyses are indicated. Asterisks indicate significant differences and correspond with P-values; *P < .05, **P < .01, ***P < .005, ****P < .001. (E) RT-qPCR quantification of gsx1 and gsx2 expression in 30 hpf embryos across genotypes. The 2^−∆∆Ct method was used to calculate relative expression to the reference gene ef1a. Data are representative of two biological replicates (parent groups)

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.