ZFIN Image: Naylor et al., 2022, Fig. 4

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Fig. 4

Depletion of cd151 in zebrafish recapitulates human phenotypes. A) Histogram shows RT-PCR for scrambled gRNA-injected controls and cd151 gRNA-injected crispants. Each data point is a biological replicate (n = 9), and relative expression is to un-injected wild-type controls. All Ct values were normalised to actb expression. B) Schematic on left shows experimental set-up for zebrafish proteinuria analysis. Histogram shows the levels of proteinuria (in relative luminescence units (RLUs)) of the indicated treatments. C) Top panel shows a normal glomerulus in a 3 dpf nphs2:egfp zebrafish embryo. Bottom panel shows the same embryo with a dilated glomerulus 1 h after HBSS injection into the cardiac cavity. Histogram illustrates the measurements of capillary width in the glomeruli of embryos pre- and post-HBSS injection. D) Histogram of RT-PCR results showing the relative gene expression of the indicated genes in cd151 crispant embryos compared to stage-matched controls. All gene expression data were normalised to actb

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Pediatr. Nephrol.