Fig. 2
Fig. 2
Dhx15?/? zebrafish displayed defective definitive hematopoiesis in CHT. A?B?, WISH analysis of cmyb expression in dhx15+/+ and dhx15?/? at 3 dpf (A, A?) and 4 dpf (B, B?). C?F?, WISH analysis of mpx (C, C?), l?plastin (D, D?), hbae1 (E, E?), rag1 (F, F?) expression in dhx15+/+ and dhx15?/? zebrafish. G?I?, WISH analysis of cmyb expression in dhx15+/+ and dhx15?/? zebrafish at 36 hpf (G, G?), 48 hpf (H, H?), 60 hpf (I, I?). Black arrows indicate CHT; red arrows indicate ventral wall of dorsal aorta; red arrowheads indicate thymus. CHT, caudal hematopoietic tissue; dpf: days post fertilization; hpf, hours post fertilization; WISH, whole?mount in situ hybridization