ZFIN Image: Tian et al., 2022, Fig. 7

Image

Figure Caption

Fig. 7

SHOX restores chondrogenesis in WS.

a, b, d, e qRT-PCR measurement of the expression of SOX9 and COL2A1 among CTR, shWRN1#, and rescue groups during chondrogenesis on day 14 in hESCs and hMSCs. N = 3 independent biological experiments. c, f Representative immunofluorescent staining of 3 independent experiments among CTR, shWRN1#, and rescue groups on day 14 in hESCs and hMSCs. SOX9 and COL2A1 were examined. Scale bar = 50 µm. g, i Dot graph analysis of the total body length among WT, wrn^−/− mutant, and rescue groups on 14 dpf (h) and 21 dpf (i). N = 25 independent zebrafish embryos for WT, wrn^−/− mutant, and rescue groups, respectively. Each dot represents a biological replicate. h, j Representative bright-field images of 3 independent experiments among WT, wrn^−/− mutant, and rescue groups on 14 dpf (h) and 21 dpf (j). Scale bar = 100 µm. k, r qRT-PCR measurement of genes (sox9a, col2a1a, and col10a1a) on 14 dpf (k) and 21 dpf (r). N = 3 independent biological experiments. l–q and s–x Representative FISH and BrdU analysis of 3 independent experiments of chondrogenic markers (sox9a, col2a1a, and col10a1a) on 14 dpf (l–q) and 21 dpf (s–x). Scale bar = 100 µm. Data are presented as the mean ± S.D. Statistical analysis was performed using two-tailed unpaired Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.