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Fig. 2
Necab2 is expressed in subsets of glutamatergic and GABAergic neurons. (A?A??) Confocal live imaging of the transgenic fish Tg(Pnecab2:EGFP) crossed with Tg(vglut2a:DsRed) at 5 dpf. Necab2 was expressed in subsets of glutamatergic neurons in the telencephalon, olfactory bulb, and olfactory epithelium (arrowheads, dorsal views). Scale bar = 50 ?m (A). The region in the dashed white box (A) was shown at higher magnification on the right (A??A??). Scale bar = 20 ?m. (B?B??). Necab2 was expressed in subsets of glutamatergic neurons in the optic tectum and cerebellum (arrowheads, lateral views). Scale bar = 50 ?m (B). The region in the dashed white box (B) was shown at higher magnification on the right (B??B??). Scale bar = 20 ?m. (C?C??) Necab2 was expressed in subsets of glutamatergic neurons in the hindbrain (arrowheads, dorsal views). Scale bar = 50 ?m (C?). The region in the dashed white box (C) was shown at higher magnification on the right (C??C??). Scale bar = 20 ?m. (D?D??) Confocal live imaging of the transgenic fish Tg(Pnecab2:EGFP) crossed with Tg(gad1b:DsRed) at 5 dpf. The Necab2-expressing neurons rarely overlapped with the gad1b-positive neurons in the telencephalon, olfactory bulb, or olfactory epithelium (arrowheads, dorsal views). Scale bar = 50 ?m (D). The region in the dashed white box (D) was shown at higher magnification on the right (D??D??). Scale bar = 20 ?m. (E?E??) Necab2-expressing neurons strongly overlapped with gad1b-positive neurons in the cerebellum (arrowheads, lateral views). Scale bar = 50 ?m (E). The region in the dashed white box (E) was shown at higher magnification on the right (E??E??). Scale bar = 20 ?m. (F?F??) Necab2-expressing neurons overlapped with gad1b-positive neurons in the hindbrain (arrowheads, dorsal views). Scale bar = 50 ?m (F). The region in the dashed white box (F) was shown at higher magnification on the right (F??F??). Scale bar = 50 ?m. (G?G??) Co-immunofluorescent staining of the anti-Necab2 with anti-SV2 in 7 dpf larvae showed that Necab2 was enriched in the pre-synaptic structures (arrowheads). Scale bar = 20 ?m (G). The region in the dashed white box (G) is shown at higher magnification on the right (G??G??). Scale bar = 10 ?m. (H?H??) Co-immunofluorescent staining of anti-Necab2 with anti-pan MAGUK in the 7 dpf larvae showed that Necab2 was enriched in the postsynaptic structures (arrowheads). Scale bar = 20 ?m (H). The region in the dashed white box (H) was shown at higher magnification on the right (H??H??). Scale bar = 10 ?m. dpf, day post-fertilization; Tel, telencephalon; Ce, cerebellum; Hb, habenula; OT, optic tectum; IPN, interpeduncular nucleus.