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Fig. 5
Figure 5. Htr2aa knockout in zebrafish attenuated the melanogenesis promotion of 5-HT and HTR2A agonist: (a) The morphology of melanocytes in zebrafish embryos at 60 hpf treated with 5-HT (1 mM) and DOI (100 μM) in wild-type siblings and htr2aa knockout zebrafish line htr2aacpu5 from 35–60 hpf. The left column shows the whole view of zebrafish embryos (lateral view). The right three columns show the amplification photos of head (lateral view), trunk (lateral view), and head (dorsal view). Scale bar, 100 μm; (b,c) relative melanin content in zebrafish embryos with or without htr2aa treated with 5-HT (b) (n = 3) or DOI (c) (n = 6); *** (p < 0.001) compared to control group; * (p < 0.05) and ns (no significance) compared to htr2aa−/− zebrafish; ### (p < 0.001) compared to wild type zebrafish with 5-HT or DOI treatment. (d) the whole mount in situ hybridization to show the expression of melanin synthesis genes, tyr, dct and tyrp1b, in wild-type siblings and htr2aa knockout zebrafish line htr2aacpu5 at 36 hpf treated with or without DOI (100 μM). Scale bar, 50 μm.
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