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Fig. 5 M and JD effects on col10a1 expression in mid-to-late time development. Shows the effects of AM6545 and JD5037 on gene expression for 62 (A,B,C) and 72 hpf (D?I) at the opercle bone marked with a black arrow. Zebrafish were exposed to the compounds from 36 to 62 hpf for osx (B,C) and 36?72 hpf for col10a1 and sparc, respectively. AM6545 (B) and JD5037 (C) increased expression of osx at 62 hpf as compared to the control (A) at 62 hpf. Both compounds (E,F) showed an increase in col10a1 expression at 72 hpf as compared to the control (D) at 72 hpf. Additionally, AM6545 (H) and JD5037 (I) increased sparc expression as compared to the control (G) at 72 hpf. n = 10 for each treatment group. For each treatment, 100% of zebrafish (B,C,E,F,H,I) showed an increase in gene expression as compared to their control (A,D,G) regardless of the gene being observed.
Reprinted from Molecular and Cellular Endocrinology, 529, Fraher, D., Mann, R.J., Dubuisson, M.J., Ellis, M.K., Yu, T., Walder, K., Ward, A.C., Winkler, C., Gibert, Y., The endocannabinoid system and retinoic acid signaling combine to influence bone growth, 111267, Copyright (2021) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.