Fig. 2

Fig. 2 Fig. 2. Growth trajectories and gene expression in gpr101 KO zebrafish. A) Standard length and body weight were measured at 3, 6, and 9 wpf in WT (black), heterozygous (HET, purple), and full gpr101 KO (blue) fish. The experiment was performed in two independent sets of fish and the data presented together. The number of fish for each genotype at all time points is given in Supplementary Fig. 7a. Data were analyzed by a two-way ANOVA; main effect of genotype, p < 0.0001 for all variables. b) Representative images of 9 wpf female fish belonging to the three genotypes. The way standard length (distance snout-caudal peduncle) was measured is shown as a horizontal white dashed line in the upper panel. The vertical black dashed line in the upper panel shows where deeply anesthetized fish were decapitated for gene expression experiments. c) The mRNA expression of a transcription factor involved in pituitary development (pou1f1), a GH-dependent growth regulator (igf1a), and pituitary and hypothalamic hormones (the rest), was measured by RT-qPCR at a juvenile stage (6 wpf) in WT (n = 11 for prl and pou1f1, n = 6 for the rest), HET (n = 17 for prl and pou1f1, n = 11 for the rest), and KO (n = 11 for prl and pou1f1, n = 6 for the rest), HET (n = 6 for prl and pou1f1, n = 5 for the rest). actb1 was used as endogenous control. None of these comparisons showed a significant difference between genotypes. GOI: gene of interest.