Fig. 3

Fig. 3

Altered genes and pathways in keap1a- and keap1b-knockout larvae.

(A) Venn diagrams showing up- (left) and down-regulated genes (right) in 5-dpf larvae of keap1a^it302-and keap1b^it308-homozygous knockout larvae and of wild-type AB larvae treated with 40 μM sulforaphane identified by an RNA-seq analysis in comparison with untreated AB larvae.

(B) A GO enrichment analysis of biological processes in keap1a- and keap1b-knockout larvae. Red, blue, green and black bars indicate the processes enriched in only keap1a-knockout larvae, both keap1a- and keap1b-knockout larvae, both keap1a-knockout and sulforaphane-treated AB larvae, and all three larvae, respectively.

(C) The comparison of the basal expression of Nrf2 target genes between wild-type and Keap1 knockout larvae. The expression of prdx1 and gstp1 in wild-type AB, keap1a- and keap1b-knockout larvae at 5 dpf was analyzed by qPCR. The expression in wild-type specimens was normalized to 1. Each experiment was conducted at least four times with duplicate samples. Asterisks indicate significant differences (*p < 0.05, ***p < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)