ZFIN Image: Rothschild et al., 2020, Fig. 8

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Figure Caption

Fig. 8 Morphological defects in camk2g1 CRISPR embryos. 4 gRNAs were designed against camk2g1 to generate G0 camk2g1 CRISPR embryos (A). G0 camk2g1 CRISPR embryos show variable phenotypes with shortened anterior posterior axis and tail curvatures evident at 48hpf (C) compared to control embryos (B). Kidney cysts were absent in control embryos (D) and developed by 48hpf in CRISPR embryos (E, asterisks). Otoliths were smaller in camk2g1 CRISPR embryos compared to controls (F, G). qPCR of CaMKII genes in camk2g1 CRISPR embryos at 24hpf relative to ef1α (H). Dorsal, anterior to the left (D, E). Lateral, anterior to the left (F, G). * p < 0.05.

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Acknowledgments

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Reprinted from Gene, 742, Rothschild, S.C., Ingram, S.R., Lu, F.I., Thisse, B., Thisse, C., Parkerson, J.A., Tombes, R.M., Genetic compensation of γ CaMKII, an evolutionarily conserved gene, 144567, Copyright (2020) with permission from Elsevier. Full text @ Gene