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Fig. 6 ECs in pPAAs are absent in lzr mutants. (A-D) IHC for mCherry (red) and EGFP (green) in WT and lzr mutant embryos with the kdrl:mCherry (ECs) and myl7:EGFP (CMs) transgenes at 72 and 80 hpf. Views in A and B are lateral with anterior left. Views in C and D are ventral with anterior up. Numbers designate the PAAs. ORA, opercular artery. White arrows and brackets indicate anterior arches. (E,F) ISH for tie1 at 36 hpf. Views are lateral with anterior left. WT (n=17), lzr (n=9). Asterisks denote aggregates of EC progenitors within developing posterior arches. White line marks tie1+ cells that extend to the dorsal aorta in lzr mutants. Scale bars: 100 µm.