ZFIN Image: He et al., 2020, Figure 1

Image

Figure Caption

Figure 1 Overexpression of <styled-content toggle='no' style='fixed-case'>FLT</styled-content>3/<styled-content toggle='no' style='fixed-case'>ITD</styled-content> induced axis duplication and ectopic expression of <styled-content toggle='no' style='fixed-case'>FST</styled-content> in zebrafish embryos

A–D

The morphology of uninjected, FLT3/WT mRNA, and FLT3/ITD mRNA‐injected (150 ng per embryo) embryos on day 2 post‐fertilization (dpf).

E–H

Whole‐mount in situ hybridization (WISH) of notochord‐specific marker col9a2 in uninjected, FLT3/WT mRNA, and FLT3/ITD mRNA‐injected embryos on 2 dpf.

I, J

FLT3 signaling was detected by Western blotting in 293FT cells transfected with FLT3/ITD mRNA (I) or in zebrafish embryos injected with FLT3/ITD mRNA (J).

K

The effect of FLT3 inhibitor quizartinib (Qui) on the dorsalization and axis duplication phenotype induced by FLT3/ITD mRNA injection in zebrafish.

L–N

Quantification of fst expression by RT–qPCR (L), Western blotting (M), and WISH (N) after FLT3/ITD overexpression in zebrafish embryos at 6 hpf.

Data information: ov: otic vesicles; cm: cephalic mesoderm; nc: notochord. Scale bar = 500 μm. In (K and L), the experiments were performed in triplicates and the data are presented as mean ± SEM. *P < 0.05 and **P < 0.01 (Student's t‐test). NS, not significant.Source data are available online for this figure.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.