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Fig. 3
Loss of prmt5 disrupts primordial germ cell migration and causes a male-like gene expression profile during sexual differentiation. (A) Vasa in embryos at the 18-somite stage (18 s), prim-5 stage (prim5) and 54 hpf. n=80 for each stage. Arrowheads indicate the cells with relative higher gene expression. (Ba) Mean numbers of ectopic PGCs in prmt5+/+ and prmt5−/− zebrafish embryos at the 18-somite stage, prim-5 stage and 54 hpf. (Bb) Scatterplot of PGC numbers in prmt5+/+ and prmt5−/− zebrafish embryos at 18-somite stage, prim-5 stage and 54 hpf. Differences between two groups were determined by unpaired two-tailed Student's t-test. ns, not significant. *P<0.05; **P<0.01; ***P<0.001. (C) vasa, amh, cyp19a1a and sycp3 in the gonads of prmt5−/− and prmt5+/+ zebrafish during sexual differentiation (30 dpf). (a-d) Male gonads; (e-h) female gonads. Red arrowheads indicate gene expression. (b,c) Upregulation of amh and downregulation of cyp19a1a in gonads of prmt5+/+ males. (f,g) Downregulation of amh and upregulation of cyp19a1a in gonads of prmt5−/− females. (j,k) Upregulation of amh and downregulation of cyp19a1a in gonads of prmt5−/− zebrafish. (d,h) Vigorous meiosis indicated by sycp3 staining in gonads of prmt5+/+ zebrafish. (i) There are few signs of meiosis in gonads of prmt5−/− zebrafish. n=6 for each staining. Scale bars: 100 μm (A); 50 μm (C).