ZFIN Image: Monestime et al., 2019, Fig. 8

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Fig. 8

MZrcor1 mutants have increased pro‐neural gene expression. A‐F. RNA in situ hybridization of MZrcor1 mutants and related wild‐type controls revealed that her6, has decreased expression pattern in the mutants at 13, 24, and 36 hpf. At 13 (G,J) and 24 hpf (H,K), ngn1, expression is more robust in the hindbrain of MZrcor1 mutants when compared to wild‐type control, while MZrcor1 mutants have increased zash1a expression at 36 hpf (N,P) compared to wild‐type controls. Quantification of expression domain at 13 hpf of her6 indicated that MZrcor1domain was 0.6 ± 0.06 µm in comparison to 1.11 ± 0.03 for wild type. Expression at 36 hpf of zash1a expression showed increased width in the MZrcor1 mutants (69.78 ± 3.5) when compared to controls (38.6 ± 2) (Q) (*P < .05) (scale bar = 20 µm). hpf, hours postfertilization

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Acknowledgments

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