ZFIN Image: Breuer et al., 2019, Fig. 5

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Figure Caption

Fig. 5

Expression of astroglial markers at 72hpf.

(A) Lateral views with anterior to the left. WISH of the BH₄ de novo synthesis pathway initiator and dopaminergic neuron marker, gch1, shows unchanged staining upon qdprb1knockdown. (B) RT-qPCR analysis reveals strongly reduced expression of glula in qdprb1 hypomorphic embryos, which is rescued by qdprb1 mRNA co-injection. (C) Lateral views with anterior to the left. This finding is corroborated by WISH experiments highlighting that glula expression is lost in the eye (asterisk in inset) and reduced in the mid/hindbrain (asterisk). (D) Also gfap expression is reduced by qdprb1 knockdown and can be rescued by qdprb1 mRNA co-injection. (E) RT-qPCR analysis of astrocytic glutamate transporters show an almost complete loss of slc1a2a, which is confirmed by WISH (F)–dorsal views focused on the eye. Asterisk indicates the effect on slc1a2aexpression. Slc1a2a expression is normalized in qdprb1 mRNA co-injected embryos (E). (G) Lateral views with anterior to the left. WISH of slc1a2b reveals mildly reduced staining in midbrain and eye (inset), confirming that both SLC1A2 homologues are affected in Qdprb1 hypomorphic embryos.

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Acknowledgments

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