Fig. 4
WISH distribution analysis of zic3 in peli1bknockdown embryos at 14 hpf. Lateral view of zebrafishembryos (A–C). Anterior view (D–F). Posterior view (G–I). WT embryos (A, D, & G), 5′ mismatch controls (B, E, & H), and peli1b morphants injected with 10 ng morpholino at the 1-cell stage (C, F, & I). Red arrowheads show zic3expression and intensity in WT, 5′ mismatch, and peli1bmorphants. (n = 3). Abbreviations: pd- posterior diencephalon, dsc- dorsal spinal cord, dnp- dorsal neural plate, hb- hindbrain, mb- midbrain, dpnp-dorsal posterior neural plate, sp-segmental plate. Scale bar- 50 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Reprinted from Gene, 694, Kumar, A., Anuppalle, M., Maddirevula, S., Huh, T.L., Choe, J., Rhee, M., Peli1b governs the brain patterning via ERK signaling pathways in zebrafish embryos, 1-6, Copyright (2019) with permission from Elsevier. Full text @ Gene