ZFIN Image: Keskin et al., 2019, Fig. 3

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Figure Caption

Fig. 3

Gene Regulatory Network in the Anterior PSM Is Mapped

(A) Wild-type (WT) embryos were continuously treated in DAPT or DMSO and fixed at different time points.

(B–D) ISH pictures for (B) mespaa, (C) mespba, and (D) her7genes in DAPT- or DMSO-treated WT embryos. These experiments were repeated twice, and 38–52 embryos were analyzed for all conditions, probes, and stages.

(E) Embryos from different genetic backgrounds were fixed immediately after 60 min of heat shock at 37°C.

(F and G) ISH images of the expression pattern of her7and deltaC immediately after 1-h heat shock in (F) hsp70l:mespab-myc or (G) hsp70l:mespbb-myc embryos.

(H and I) ISH images of mespba or mespaa expression immediately after 1-h heat-shock in (H) hsp70l:mespab-myc or (I) hsp70l:mespbb-myc embryos. (F–I) These experiments were repeated twice, and 50–65 embryos were evaluated for all genotypes, probes, and stages.

Acknowledgments

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