ZFIN Image: Gutzman et al., 2018, Fig. 2

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Fig. 2

Wnt5b regulates basal constriction possibly through Gsk3?. (A?D) In situ hybridization of wnt5b expression during MHB development at 18?ss (A), 22?ss (B), and prim-6 (C). (D) prim-6 sense probe control. (E?G?) Live confocal images of the MHB region in prim-6 stage embryos. Single-cell wild-type embryos were injected with mGFP to label cell membranes and co-injected with control MO (E,E?), wnt5b MO (F,F?), or wnt5b MO and dnGsk3? mRNA (G,G?). (H) Quantification of basal cell width in control MO, wnt5b MO, dnGsk3? mRNA (image not shown), and wnt5b MO+dnGsk3? mRNA injected embryos. (H) For each treatment group, n=3 embryos. For each embryo, 6 cells located at the MHBC were measured, 3 cells on each side. Arrowheads indicate MHBC. M, midbrain; H, hindbrain. Scale bars: 26?�m.

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Acknowledgments

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