ZFIN Image: Kinoshita et al., 2018, Fig. S4

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Fig. S4

Somite segmentation defects in ripply1 homozygous embryos were rescued by partial knockdown of tbx6. (A) Lateral views of uninjectedand low-dose tbx6 MO-injected ripply1-/- embryos at the 20-somite stage. tbx6MO was injected at a low dose (concentration of 0.5 mg/ml) into blastomeresof fertilized embryos obtained by intercross between ripply1homozygous fish. (B) Immunohistochemistry of the myotomes of uninjectedand low-dose tbx6MO-injected ripply1-/-embryos at 36 hpfusing MF20 and anti-Mef2c antibodies. The arrowhead shows the posterior end of the yolk extension. Anterior is to the left. (C) Representative images of uninjectedembryos and low-dose tbx6MO-injected ripply1-/-embryos at 36 hpf. The somite boundary was visualized by in situstaining with xirp2a/cb1045 antisense probe. (D) Distribution of somite boundary formation in uninjectedripply1-/-and tbx6 MO-injected ripply1-/-embryos. In the low-dose tbx6MO-injected embryos, the stained embryos showing recovery of the somite boundary were examined. See Materials and Methods for the details of this analysis. Scale bars in A, B, and C are 100 mm.

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Acknowledgments

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Reprinted from Mechanisms of Development, 152, Kinoshita, H., Ohgane, N., Fujino, Y., Yabe, T., Ovara, H., Yokota, D., Izuka, A., Kage, D., Yamasu, K., Takada, S., Kawamura, A., Functional roles of the Ripply-mediated suppression of segmentation gene expression at the anterior presomitic mesoderm in zebrafish, 21-31, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.