Fig. S5
Injection of gdf3 mRNA into tbx1 mutants is not sufficient to rescue nkx2.5 expression at 6 somite stage, Related to Figure 5.
(A-F) Brightfield images of 4.7 hours post-fertilization (hpf) (A-C) or 6 somite stage (ss) (D-F) uninjected control (CTRL; A, n=28; D, n=29) and tbx1 mutant (B, n=7; E, n=8) embryos or tbx1 mutant embryos injected with gdf3 mRNA at the one-cell stage (C, n=6; F, n=8). Embryos were processed by in situ hybridization with no tail (A-C) or nkx2.5 riboprobes (D-F). The asterisk in (C) highlights ectopic mesoderm induced by gdf3 mRNA. Lateral views in (A-C). Dorsal views, anterior up in (D-F). Little to no variation was observed between animals in each experimental group. Scale bars=25μm.