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Fig. S3
Rspo1 knockdown does not alter primary vascular specification. Wild-type (A,C,E,G) or Rspo1 MO injected animals (B,D,F,H) were examined by WISH for expression of endothelium (cdh5, A,B), arterial identity (dlc, C,D and efnb2a, E,F), or by fluorescence for vessel patterning (fli1a:EGFP, G,H) at the time points indicated, revealing normal patterning. (H) Rspo1 morphants have stunted intersegmental vessel growth at 26 hpf. Numbers of embryos displaying the depicted phenotype are indicated. Magnification 160X.
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