ZFIN Image: Selland et al., 2018, Fig. 4

Image

Figure Caption

Fig. 4

Hoxb1b^−/−;pbx4^−/− double mutants revert to the r1-like ground state.

Eng2 expression in the midbrain-hindbrain boundary is observed in all embryos (A-D), however krox20 and epha4a expression are altered. As previously observed, hoxb1b^−/− mutants have an expanded r3 domain and a reduced r4 domain (6/6 embryos) (B, F). In pbx4^−/− mutants r3 is drastically reduced (12/12 embryos)(C,G), and in hoxb1b^−/−;pbx4^−/− double mutants all krox20 expression in r3 and r5 is lost (3/3 embryos)(D), while the r1 domain of epha4a expression is expanded posteriorly throughout the hindbrain (3/3 embryos)(H). All embryos have been genotyped for pbx4^b557 and hoxb1b^ua1006. All images are dorsal views, anterior to the left, 22hpf.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 150, Selland, L.G., Koch, S., Laraque, M., Waskiewicz, A.J., Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development, 28-41, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.