ZFIN Image: Williams et al., 2018, Fig. S8

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Figure Caption

Fig. S8

Calyculin A treatment increases notochord boundary tension without improving MZudu-/- boundary straightness or cell polarity

a) Quantification of cell vertex recoil distance upon laser ablation of Edge cell interfaces at the time points indicated in MZudu-/-, WT, and Calyculin A-treated WT gastrulae. Symbols are means with SEM (2-way ANOVA, ***p=0.0006). b) Quantification of boundary straightness in WT and MZudu-/- gastrulae with or without Calyculin A treatment. Symbols are means with SEM (2-way ANOVA, ****p<0.0001). c-d) Cell vertex recoil distance upon laser ablation of Edge cell interfaces at the time points indicated in WT embryos overexpressing itga3b (c) or epcam (d) with or without Calyculin A treatment. Symbols are means with SEM (2- way ANOVA, **p=0.027, ****p<0.0001). e-h) Quantification of axial mesoderm cell orientation (g) and elongation (h) in Calyculin A-treated MZudu-/- gastrulae. Graphs and color coding as in Fig.3. Bars are median values in g and mean values in h. Scale bar is 50μm. N indicates the number of embryos analyzed.

Acknowledgments

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