|
Fig. 4
Metformin reduces leukocyte infiltration in pkd2 morphants. (A) Representative images of l-plastin-positive and mpx-positive cells surrounding the pronephric ducts in a control MO-injected embryo, a pkd2 morphant, and a pkd2 morphant treated with metformin (10 mM). Leukocytes were labelled using in situ hybridisation for l-plastin and mpx at 48 hpf. The lower panel shows an enlarged view of the dashed box indicated in the upper image. Scale bar, 200 µm. (B) Counts of the l-plastin-positive cells (within the region of yolk extension as indicated by the dashed box) in the controls and metformin-treated pkd2 morphants (2.5 mM to 20 mM). ***P < 0.001 compared with pkd2 morphants. Data represent two independent experiments, n = 7–11 per group. (C) Counts of the mpx-positive cells. **P < 0.01, ***P < 0.001 compared with pkd2 morphants. n.s. = not significant. Data represent two independent experiments, n = 16–20 per group.