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Fig. 3
Expression of Prg4 genes in articular chondrocytes of ray-finned fish.
(A–G) prg4b expression in articular chondrocytes of the zebrafish jaw joint (boxed region in A, B–D), hyoid joint (E); ray-scapula joint (F); and vertebral column (G). prg4b is also expressed in the liver (asterisk), possibly in ligaments above the vertebrae, and weakly at the ceratohyal-ceratohyal joint (arrowhead). n = 3 each. (H–J) Expression of stickleback prg4b (1 mpf, n = 3) and gar prg4 (10.2 cm, n = 3) in jaw joint articular chondrocytes (J, magnification of I). (K–M) Exclusion of acana, col10a1, and matn1 expression from articular chondrocytes of the zebrafish jaw. n = 3 each. Scale bar, 50 μm. ih: interhyal. See also Figure 3—figure supplement 1 and 2.